EXPLORATION OF A HOT SPRING FOR THERMOSTABLE PROTEASE PRODUCERS

Thermophilic Bacillus Licheniformis Rbs 5 Isolated from Hot Tunisian Spring Co-producing Alkaline and Thermostable Α-amylase and Protease Enzymes

Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran

Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic a...

Production and Purification of a Thermostable Lipase from a Hyperactive Bacterial Isolate Brevibacillus Sp. from Taptapani Hot Spring, Odisha

Thermostable lipase producing Brevibacillus sp. was isolated from Taptapani hot water spring of Ganjam, Odisha, India. Maximum lipase production by Brevibacillus sp. was obtained at pH 10 when grown under shaking condition at 60 °C for 30 hrs. To enhance the production of lipase, nutritional factors were optimized and it was observed that maximum lipase production was obtained in the presence o...

Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach

The DNA extracted from a high-temperature environment in which micro-organisms are living will be a good source for the isolation of thermostable enzymes. Using a metagenomic approach, we aimed to isolate thermostable β-xylosidases that will be exploited for biofuel production from lignocellulosic biomass. DNA samples obtained from the soil near a spout of a hot spring (70°C, pH7.2) were subjec...

isolation and characterization of a thermophilic bacillus sp. with protease activity isolated from hot spring of tarabalo, odisha, india.

background and objectives: thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. materials and methods: in this study, thermophilic bacteria were isolated from hot spring of tarabalo, india. a bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16s rrna gene...